Sutter Manual Microinjector
Manual Microinjector
The Manual Injector is a manual syringe driver for pneumatic or
hydraulic control of injection needles or holding pipettes. It is
suitable for injecting volumes in the nanoliter to microliter range. It
is also widely used as a fluid control device for IVF applications
requiring sensitive manual control of the displacement of micro needle contents.
The system is constructed from the highest quality parts. The base
assembly is black anodized aluminum. A non-rotating Mitutoyo micrometer
provides the drive to the gas-tight syringe1 . A
precision 3-way valve provides a convenient method for filling the fluid
line and clearing air bubbles from the line. Teflon tubing,
chromatography connectors and a pipette holder2
complete the system.
The injection resolution is dependent on the volume
of the syringe that is installed, as outlined in the following table.
For example, with a 25µl syringe installed, one complete rotation of
the micrometer (25 divisions) yields a displacement equivalent to a
volume of 267nl and turning the micrometer one division (0.001in) yields
10.6nl.
| Syringe Volume |
Volume per Revolution |
Volume per Division |
| 10 µl |
106 nl |
4.2 nl |
| 25 µl |
267 nl |
10.6 nl |
| 50 µl |
529 nl |
21.2 nl |
| 100 µl |
1.06 µl |
42.3 nl |
| 250 µl |
2.65 µl |
105.8 nl |
| 500 µl |
5.29 µl |
211.7 nl |
| 1000 µl |
10.58 µl |
423.4 nl |
1) Please specify the syringe volume that you will be using. For IVF
applications, 500µl and 1000µl syringes are commonly used.
2) Unless specified otherwise, a pipette holder for 1mm outside
diameter glass will be supplied. The diameter of the pipette holder is
0.25 inches (6.4mm). For micromanipulators that cannot accept this size
holder, an adapter is optionally available. Please contact us for
further information.
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Site by Rob Jones.
Copyright © 1999 Linton Instrumentation . All rights reserved.
Revised: October 30, 2002
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